It was another ugly match against Charlotte as the Lions finish 0-2-1 in three games against the North Carolina side.
Facundo Torres opened the scoring and then broke a 1-1 tie late from the penalty spot as Orlando City beat the Western Conference leaders.
The Lions roared back from 1-0 down and became just the second team to beat the Fire at Soldier Field this season.
A tie score from halftime was broken by yet another soft penalty given to an Orlando opponent and the Lions are done with Leagues Cup.
Wilder Cartagena's stoppage-time goal snapped a 2-2 deadlock and lifted the Lions into the Round of 32.
The Lions conceded a highly questionable penalty at the end of the first half, then battled back to equalize and won the postgame shootout.
Antonio Carlos and Duncan McGuire led the Lions to a comeback victory in Atlanta.
After conceding a pair of goals on first-half set pieces, the Lions never mounted any kind of challenge.
For the second straight year, the Lions dominated Toronto FC at home, winning 4-0.
The Lions made it six in a row at home without a loss with a win over the Fire.
Isnt Latte Lath suspended this weekend for 5 yellow card accumulation?